The present paper provides an updated checklist of the ants (Hymenoptera: Formicidae) of Pakistan. These include seven of the 21 known extant subfamilies with 101 valid ant species in 33 genera. Five species are reported for the first time from Pakistan: Cardiocondyla wroughtonii Forel. 1890; Crematogaster biroi Mayr. https://www.mabelandfoxs.shop/product-category/silicone-mat/